TRAK1 Knockout cell line (HEK293)

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location： Home > Products > KO Cell Lines > TRAK1 Knockout cell line (HEK293)

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TRAK1 Knockout cell line (HEK293) Order

TRAK1 Knockout cell line (HEK293)
Catalog#
YKO-XN48122
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TRAK1 Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TRAK1 Knockout cell line (HEK293)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
TRAK1
Gene ID
22906
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 4 (based on transcript TRAK1-201)
Validation: PCR+Sanger Sequencing
TRAK1 Knockout cell line (HEK293) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HEK293		 STR Info (Cell bank)
Cell Line:HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
D12S391 1921
FGA 2323
D2S1338 1919
D21S11 2830.2
D18S51 1718
D8S1179 1214
D3S1358 1517
D6S1043 1111
PENTAE 715
D19S433 1818
PENTAD 910
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TRAK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TRAK1 gene knockout TRAK1 Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TRAK1 Knockout cell line (HEK293)
TRAK1 Knockout cell line (HEK293)
Catalog#
YKO-XN48122
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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EZ-cryo™ cell freezing medium

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  • TRAK1 Knockout cell line (Hep G2)

    TRAK1 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-HT4688
    YKO-HT4688(Hep G2)
  • TRAK1 Knockout cell line (HeLa)

    TRAK1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-H1115
    YKO-H1115(Hela)
  • TRAK1 Knockout cell line (HCT 116)

    TRAK1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-XN48123
    YKO-XN48123(HCT 116)

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    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TRAK1 Knockout cell line (Hep G2)

    TRAK1 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-HT4688
      inquiry   More details
  • TRAK1 Knockout cell line (HeLa)

    TRAK1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-H1115
      inquiry   More details
  • TRAK1 Knockout cell line (HCT 116)

    TRAK1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-XN48123
      inquiry   More details

TRAK1 Knockout cell line (HEK293) Order

TRAK1 Knockout cell line (HEK293)
Catalog#
YKO-XN48122
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TRAK1 Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TRAK1 Knockout cell line (HEK293)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
TRAK1
Gene ID
22906
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 4 (based on transcript TRAK1-201)
Validation: PCR+Sanger Sequencing
TRAK1 Knockout cell line (HEK293) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HEK293		 STR Info (Cell bank)
Cell Line:HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
D12S391 1921
FGA 2323
D2S1338 1919
D21S11 2830.2
D18S51 1718
D8S1179 1214
D3S1358 1517
D6S1043 1111
PENTAE 715
D19S433 1818
PENTAD 910
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TRAK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TRAK1 gene knockout TRAK1 Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TRAK1 Knockout cell line (HEK293)
TRAK1 Knockout cell line (HEK293)
Catalog#
YKO-XN48122
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TRAK1 Knockout cell line (Hep G2)

    TRAK1 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-HT4688
    YKO-HT4688(Hep G2)
  • TRAK1 Knockout cell line (HeLa)

    TRAK1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-H1115
    YKO-H1115(Hela)
  • TRAK1 Knockout cell line (HCT 116)

    TRAK1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-XN48123
    YKO-XN48123(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TRAK1 Knockout cell line (Hep G2)

    TRAK1 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-HT4688
      inquiry   More details
  • TRAK1 Knockout cell line (HeLa)

    TRAK1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-H1115
      inquiry   More details
  • TRAK1 Knockout cell line (HCT 116)

    TRAK1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TRAK1
    Gene ID: 22906
    Size: 1*10^6
    Catalog#: YKO-XN48123
      inquiry   More details

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